Nucleic Acid/Protein Concentration: An Analysis of the Multi-functional Applications of Ultramicro Spectrophotometer

In molecular biology experiments, the quantification of nucleic acids and proteins is a fundamental yet critical step. Traditional spectrophotometers typically require larger sample volumes and involve cumbersome operational procedures. In contrast, ultramicro spectrophotometers require only 0.5 to 2 μL of sample and can complete measurements within 6 seconds, with a detection range spanning from 2 to 15,000 ng/μL. How does this device—integrating multi-path detection, fluorescence quantification, and an intelligent operating system—achieve such high efficiency? Let us explore its underlying mechanisms.

1. High-Precision Detection with Extended Light Source Lifespan 

The ultramicro spectrophotometer incorporates an innovative light source flicker algorithm. Compared to conventional methods, this design significantly prolongs the lifespan of the light source. Traditional spectrophotometers operate with continuous illumination, which not only accelerates wear and tear but may also lead to sample degradation due to prolonged exposure to intense light. The flicker algorithm minimizes light intensity exposure, thereby reducing sample degradation and enabling faster, more accurate measurements. Additionally, the light source fluctuation peak-to-peak (pp) value—defined as the difference between the maximum and minimum light intensity—is maintained below 0.5%, ensuring consistent light intensity throughout the detection process. This high level of stability supports precise and reliable measurements, which are essential in fields such as life sciences and pharmaceutical research, where accurate data acquisition is crucial for advancing scientific discovery.

2. Versatile Functionalities to Meet Diverse Experimental Needs

The ultramicro spectrophotometer is equipped with a range of advanced functionalities. Utilizing proprietary motor control technology and a four-path detection system, it achieves superior stability, repeatability, and linearity across a broader detection range. Samples do not require dilution, and the measurable concentration range exceeds that of conventional UV-Vis spectrophotometers by more than 150-fold, significantly reducing both time and cost associated with sample preparation. Furthermore, the device includes an OD600 optical density detection system based on cuvette mode, enabling efficient quantification of bacterial and microbial cultures. In fluorescence detection, when used in conjunction with fluorescence quantification kits, the device enables precise quantification of DNA, RNA, and protein concentrations through the specific binding of fluorescent dyes to target molecules. This multifunctional capability provides robust support for molecular biology research and related applications.

3. User-Friendly Interface and Efficient Data Management

The ultramicro spectrophotometer features a highly intuitive and user-friendly interface. Powered by an embedded Android operating system and equipped with a 7-inch capacitive touch screen, it operates independently without the need for external computer connectivity, thereby enhancing operational efficiency. The device also includes a built-in printer for direct report generation, streamlining data documentation. Data can be stored in both tabular and image formats, with tables compatible with Excel for seamless post-processing. JPG image export is also supported. The device operates within a wavelength range of 190–850 nm and requires only 0.5–2 μL of sample. It offers multiple optical path options—0.03 mm, 0.05 mm (for high-concentration samples), 0.2 mm, and 1.0 mm (for standard-concentration samples)—to accommodate a wide variety of sample types and concentration levels.

With its high-precision detection capabilities, multifunctional design, and user-friendly interface, the ultramicro spectrophotometer plays a pivotal role in modern scientific research and analytical testing. It provides researchers with a more efficient and accurate means of molecular quantification, thereby facilitating the advancement of scientific inquiry.