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Product Introduction
Laboratories running comprehensive multi-step assay workflows — where compound dispensing, serial dilution, secondary reagent addition and plate reformatting must occur in sequence — face a fundamental throughput limitation when instrument deck capacity requires plate swapping mid-protocol. The YA200-5 from HM Instruments eliminates this bottleneck with 5 SBS deck positions on the 5–200 μL air-displacement platform, enabling up to 5 sequential liquid handling operations in a single unattended run.
The 5-deck configuration accommodates the full workflow chain: compound source plate, diluent reservoir, intermediate dilution plate, destination assay plate and tip box. This is particularly valuable for diagnostic laboratories running comprehensive ELISA panels (sample → conjugate → substrate → stop solution), research teams performing NGS library preparation (enzymatic reaction → bead cleanup → elution → index addition), and screening labs requiring compound addition → serial dilution → secondary reagent addition in a continuous protocol.
The air-displacement pipetting delivers ≤0.8% accuracy at 200 μL and ≤0.5% CV, ensuring that multi-step protocols maintain precision through every transfer. Adjustable dispensing speed accommodates viscous DMSO stocks for compound addition and gentle dispensing for cell-based assays. The Android tablet with Bluetooth control enables flexible operation, while the compact 580 × 350 × 580 mm footprint allows installation inside biosafety cabinets for aseptic workflows.
Applications
Comprehensive ELISA panels: sequential sample, conjugate, substrate and stop solution addition
NGS library preparation: enzymatic reaction followed by bead cleanup and elution
Multi-reagent compound screening with serial dilution and secondary detection reagent
Cell-based assay protocols: compound addition → media exchange → viability reagent
Plate format reformatting from 96 to 384 wells for high-volume diagnostic testing
Key Features & Advantages
5 SBS deck positions support up to 5-step protocols without plate swapping
Multi-source reagent loading: 2+ reagent sources plus destination and tips simultaneously
5–200 μL wide volume range covers all steps from compound dispensing to buffer addition
≤0.8% accuracy at 200 μL and ≤0.5% CV maintained through multi-step transfers
Built-in gradient dilution for dose-response and calibration curve generation
96-to-384 automated reformatting reduces assay cost by 75%
Adjustable aspiration speed for DMSO stocks, glycerol and cell suspensions
Android tablet with visual drag-and-drop protocol editor
Bluetooth wireless control with BSC-internal instrument and external tablet
USB Type-C (PD) for simultaneous charging and data transfer
580 × 350 × 580 mm footprint for BSC or bench installation
Universal tip compatibility avoids proprietary consumable contracts
GBT 41812-2022 verified pipetting precision
Technical Specifications
| Parameter | Value |
|---|---|
| Pipetting principle | Air displacement |
| Number of channels | 96 (fixed) |
| Volume range | 5–200 μL |
| Accuracy | ≤1.5% at 20 μL; ≤1% at 100 μL; ≤0.8% at 200 μL |
| Precision (CV) | ≤1% at 20 μL; ≤0.8% at 100 μL; ≤0.5% at 200 μL |
| Deck positions | 5 |
| Plate format | Standard SBS |
| Control interface | Android tablet |
| Connectivity | Bluetooth, USB Type-C (PD) |
| Power supply | 180–264 VAC, 47–63 Hz, 220W |
| Dimensions | 580 × 350 × 580 mm |
| Weight | 26.6 kg |
| Compliance standard | GBT 41812-2022 |
FAQ
Q: What types of multi-step protocols benefit most from 5 deck positions?
A: Protocols requiring more than 3 distinct liquid sources or intermediate processing steps benefit most. Common examples include comprehensive ELISA panels (4 reagent additions), NGS library prep (enzymatic reaction + bead cleanup + elution + barcode addition), and compound screening with secondary detection reagent addition. These protocols require 4–5 deck positions to run unattended.
Q: How does the YA200-5 maintain precision across multi-step protocols?
A: The air-displacement pipetting system with ≤0.5% CV at 200 μL ensures that each individual transfer step maintains precision. Error does not accumulate significantly across sequential steps because each transfer is independently controlled by the motor-driven piston. In practice, 5-step protocols show final-step precision within 1% of single-step precision.
Q: Can the 5-deck configuration accommodate both 96-well and 384-well plates?
A: Yes. All 5 deck positions accept standard SBS-format plates, including 96-well, 384-well and deep-well plates. The 96-to-384 reformatting function transfers from a 96-well source on one deck position to a 384-well destination on another, enabling format conversion within multi-step protocols.
Q: Is the YA200-5 suitable for clinical diagnostic labs?
A: Yes. The 5-deck configuration supports comprehensive ELISA workflows (sample → conjugate → substrate → stop) in a single unattended run. The compact footprint fits inside biosafety cabinets, and the Android tablet interface with sample mapping reduces transposition error risk. GBT 41812-2022 compliance provides traceability documentation for regulatory audits.
Q: What is the service and support coverage?
A: HM Instruments provides 280 service centers across China with 24-hour response and 4-hour on-site arrival in provincial capitals. The instrument carries a 12-month warranty covering the entire system. Lifetime technical support and free software upgrades are included. For international customers, remote technical support is available.
Address of this article:https://www.labinstruments.net/automated-pipetting-workstation/wide-range-pipetting-comprehensive-assay-workflow.html
